Small RNA interfering effects in chicken embryonic fibroblasts using two sites 8664/p201 and 2122/pll3.7 of chicken s1p1/edg 1 gene

Introduction: Currently, short interfering RNA (siRNA) is an experimental tool in molecular biology to study gene function cell culture and vivo model of organisms (mouse chicken embryos, etc.) Method: Lentivirus production technique by human embryonic kidney 293T lines (HEK293T) with two (2) sites 8664/P201 2122/Pll.3.7 the sphingosine 1 phosphate receptor endothelial differentiation (S1P1/ Chicken EDG1) was used show effects small fibroblasts during embryogenesis. After preparation plasmids 2122/Pll3.7, lentivirus packaging, transfection 2xHBS, virus titration egg preparation, microinjection performed embryos chicken. Results: The results obtained after into from second day fourth incubation showed development materialized bleeding yolk sac therefore a decrease function. Conclusion: Previous studies have already proven that expression decreases certain vertebrates such as mammals.